B2883

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B2883

Sigma-Aldrich

 

bisBenzimide H 33258

≥98% (HPLC and TLC)

Synonym:BBIH, HOE 33258, Hoechst 33258, 2′-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi(1H-benzimidazole) trihydrochloride, 2-[2-(4-Hydroxyphenyl)-6-benzimidazoyl]-6-(1-methyl-4-piperazyl)benzimidazole trihydrochloride
CAS Number:23491-45-4
Linear Formula:C25H24N6O · 3HCl
Molecular Weight:533.88
Beilstein Registry Number:4088183
EC Number:245-690-6
MDL number:MFCD00012679
PubChem Substance ID:24891414

Description

Biochem/physiol ActionsMembrane-permeable, fluorescent DNA stain with low cytotoxicity that intercalate in A-T regions of DNA.
ApplicationUseful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry.
Excitation max. = 346 nm
Emission max. = 460 nm

Properties

assay≥98% (HPLC and TLC)
solubilityH2O: 10 mg/mL
 phosphate buffers:  precipitates
suitabilitypasses application test for fluorescence
storage temp.−20°C

Safety

Personal Protective Equipmentdust mask type N95 (US), Eyeshields, Gloves
Hazard CodesXn
Risk Statements22-36/38
Safety Statements26-36
WGK Germany3
RTECSSM1140500

Related Products

Related productMYC1, Mycoplasma Stain Kit

References

referenceAraki, T., et al. Histochemistry 87, 331, (1987) Abstract
 Latt, S.A. and Stetten, G. J. Histochem. Cytochem. 24, 24, (1976)